A Novel: Low-cost Method for the Isolation of Anaerobic Hydrogen Producing Bacteria

Abstract The hydrogen content of the two sludge samples namely TDS and PLS were increased after heat shock pretreatment from 239.23 ± 10.23 mL/L to 663.87 15.99 at 80°C 173.61 17.21 496.74 30.21 60°C, respectively. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) indicated that Enterococcus Clostridium dominant bacteria in consortium. Two strains successfully isolated using a simple method. Based on Bergey’s key identification 16S rDNA sequencing, they identified as butyricum strain TD acetobutylicum PL. content, glucose consumed yield, yield C. PL 545.24 7.31 mL/L, 90 0.41 %, 0.89 0.01 mol H 2 /mol glucose, 729.34 22.81 92.77 1.39 1.26 0.05